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KMID : 0613820080180020180
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Journal of Life Science
2008 Volume.18 No. 2 p.180 ~ p.186
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Inhibition of Phorbol 12-myristate-13-acetate Induced Cyclooxygenase-2 Activity by Three-step Fermented Soybeans
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Park Cheol
Lee Jeong-Ok
Ryu Chung-Ho
Choi Yung-Hyun
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Abstract
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In this study, we examined the effects of the fermented soybeans by Bacillus subtilis (FSB) and the novel three-step fermented soybeans (TFS) on the expression and activity of COX-2 in human leukemic U937 cell model. Treatment of phorbol 12-myristate 13-acetate (PMA) significantly induced pro-inflammatory mediators such as COX-2 expression and prostaglandin E2(PGE2) production, whereas the levels of COX-1 remained unchanged. However, pre-treatment with FSB and TFS significantly attenuated the PMA-induced COX-2 protein as well as mRNA, which was associated with inhibition of PGE2 production. Moreover, TFS exerts a much better inhibitory activity than FSB against PMA-induced activation of COX-2 and production of PGE2 in U937 cells. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-inflammatory activity of FSB and TFS.
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KEYWORD
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Cyclooxygenase-2, prostaglandin E2 , fermented soybeans by Bacillus subtilis, three-step fermented soybeans
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